Isolation, Culturing and Cryopreservation of Putative Granulosa Stem Cells from Buffalo Ovaries

Authors

  • Sesha Subramanian Department of Centre of Excellence in life Sciences, Bharathidasan University, Tiruchirappalli, TamilNadu, India Author
  • Aarti Raj Department of Biotechnology, Punjab Technical University, Ludhiana, Punjab, India. Author
  • Rajesh Kumar Department of Biotechnology, Punjab Technical University, Ludhiana, Punjab, India. Author
  • Satish Kumar Rana Department of Biotechnology Engineering, Ambala College of Engineering and Applied Research, Mithapur, Ambala, Haryana, India Author
  • Anal Kant Jha Aryabhatta Centre for Nanoscience and Nanotechnology Aryabhatta Knowledge University, CNLU Campus, Pana, India. Author
  • Sanjeev Gautam ξDepartment of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India. Author

Keywords:

Buffalo, granulosa stem cells, confluent monolayer, characterization and reproductive biotechnology

Abstract

The current study was carried out to isolate, culture, characterize and cryopreserve the granulosa stem cells from buffalo ovaries. Granulosa stem cells were cultured in DMEM containing15% FBS, 1% penicillin/ streptomycin and 1%L-glutamine in 5% CO2 with humidified air at 38.5±0.5˚C. After 3 days of culture different morphologies of cells were observed. The confluent monolayer should have been formed at the base of the droplet after 72 hrs of culture. The culture was harvested and cultured in a culture media. Followed by cryopreservation of the granulosa stem cells. This result may contribute towards establishing further studies of granulosa stem cells for various therapeutic and reproductive biotechnological applications.

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Published

2015-10-11

Issue

Vol. 4 No. 01 (2015): International Journal of Cell Science and Biotechnology

Section

Articles